Skip to content

Posts by Colin Campbell

Do Funding Agencies Favor Collaboration?

It’s an interesting question, and certainly one scientists need to think about. In a recent conversation a science colleague said, “I think in science right now, all the funding agencies are recognizing that to answer the problems that matter, you need to bring in people from different disciplines and even industry. If you look at the major funding focus of the National Science Foundation, when they consider bio-complexity, they’re not looking for a group of people with the same perspective. Science questions are becoming more complex, so you need to get input from people with varied backgrounds.”

funding

R.J. Cook Agronomy Farm at WSU (http://css.wsu.edu/facilities/cook/)

Examples of this are two projects that METER has collaborated on recently: the Specialty Crops Research Initiative – Managing Irrigation and Nutrients via Distributed Sensing (SCRI- MINDS) and the WSU Cook Farm project, both of which were able to get funding based in part on the use of METER’s technology, and both had a high-level of multidisciplinary involvement.

We got involved in the Cook Farm Project seven years ago because another scientist and I had an idea that fit in the context of a hot topic of the time which was to create a wireless sensor network that was densely populated in a relatively small area.  We did this because at that time, scientists were recognizing that many of the processes they were interested in were occurring when they were not out in the field measuring. In order to understand these processes, we needed in situ measurements collected continuously over a long period of time.

What we were trying to do is show that you could create a wireless sensor network in a star pattern, where you have a central point collecting data from a host of nodes surrounding it.  Our questions were:  can we create a sustainable star network in the field to get consistent and continuous measurements over several seasons, while densely populating the study area with sensors? The measurement network that we designed allowed us to investigate how topography, slope, and aspect interact to determine the hydrology of the soil in this intensely managed agronomic field.

Decagon collaborated with scientists at Washington State University, working with twelve sites across a 37-hectare field.  We installed five ECH2O-TE (now 5TE) sensors at 30, 60, 90, 120, and 150 cm below the soil surface.

funding

Wheat field

What we learned was that when wheat plants grow, their roots follow the water down a lot deeper than you might imagine.  We observed considerable water loss even 150 cm below the soil surface. Data on soil water potential suggested that, as water was depleted to the point where it was not easily extractable, plant roots at a given level would move deeper into the soil where water was more easily accessible. Soil morphology also came into play as hardpans occurred at several measurement locations and water uptake from layers above and below them showed amazing differences.

It was a really exciting thing scientifically, but also technologically.  We learned that the star network was easily possible.  It ran autonomously and was very successful, in spite of the fact that the cell phone we used to get the data back to the office never worked very well.

So it was the science question and the technology question together that was able to secure the funding.  With those twelve sites WSU was able to secure a grant from the USDA for 4.2 million dollars and the research is still ongoing today.  In fact, recently Cook Farm was established as one of the National long-term agroecosystem research sites (LTAR) which will help continue this kind of research well into the future.

Download the “Researcher’s complete guide to water potential”—>

Download the “Researcher’s complete guide to soil moisture”—>

Get more information on applied environmental research in our

Measuring Osmotic Sap Water Potential

Sometimes networking with new scientists at conferences and workshops can pay dividends in terms of new ideas. Steve Garrity and I recently attended and taught practicum sessions at the PEPg (Plant Environmental Physiology group) Ecophysiology Workshop. The mission of this workshop was twofold: to invite the world experts on plant physiology measurements to come and lecture, and to invite the manufacturers to teach about instrumentation and provide hands-on training.

sap

Workshop participants check the water potential of soil with a UMS T5 mini-tensiometer.

With three sessions per day using METER instrumentation and only two of us, neither Steve nor I could teach about leaf water potential using the WP4C chilled mirror dew point instrument. So, we asked another scientist who is an expert in plant water relations to teach it for us.  Not only did he do a great job of teaching about measuring leaf water potential using a hygrometer, but he also inspired us to take another look at how to make this measurement as we learned about its importance to his research (to learn more about how to do this, watch our virtual seminar).

sap

He’s developed a procedure where you can freeze the leaf and break all of the cells so you are left with the cell water (the symplastic water).

Later in the conference, this same scientist gave a talk about the importance of osmotic potential.  He’s developed a procedure where you can freeze the leaf and break all of the cells so you are left with the cell water (the symplastic water).  He was able to squeeze that sap out and test it in a thermocouple psychrometer, where he established a relationship between how tolerant plants are for drought and what their osmotic sap water potential (turgor loss point) was. We have made many of those sap measurements but had not used them in this manner. That’s really interesting to us at METER because we were unaware of this relationship, and we have now found another use for osmotic potential measurements in leaves.

We would never have realized this new idea without the help of our colleague.  Meeting with other scientists at conferences and talking over ideas can be really important.  Have you ever struck gold in terms of coming up with new ideas for research, funding, or inventing new research tools at a conference you’ve attended?

Download the “Researcher’s complete guide to water potential”—>

Download the “Researcher’s complete guide to soil moisture”—>

Get more information on applied environmental research in our

Despite Drawbacks, Scientific Collaboration Pays Off

Though collaboration can fuel innovation and increase the relevance and complexity of the scientific questions we study, I’ve noticed it does have its ups and downs.  The highs and lows we’ve run into on our research projects may help others avoid some of the pitfalls we experienced as many diverse groups tried to learn how to work together.

collaboration

Researchers discussing science at the Lytle Ranch Preserve, a remarkable desert laboratory located at the convergence of the Great Basin, Colorado Plateau, and Mojave Desert biogeographical regions.

There can be bumps in the road when collaborating with companies who want to test their product. Being at the forefront of innovation means that untested sensors may require patience as you work out all the bugs together. But from my perspective, this is part of the fun.  If we are late adopters of technology, we wouldn’t get to have a say in creating the sensors that will best fit our projects as scientists.

Collaborating scientists can also sometimes run into problems in terms of the stress of setting up an experiment in the time frame that is best for everyone.  During our experiment on the Wasatch Plateau, we had six weeks to get together soil moisture and water potential sensors, but our new GS3 water content, temperature, and EC sensors had never been outside of the lab. In addition, we planned to use an NDVI sensor concept that came out of a workshop idea my father Gaylon had.  We’d made ONE, and it seemed to work, but that is a long way from the 20 we needed for a long-term experiment in a remote location at 3000 meters elevation. In the end, it all worked out, but not without several late nights and a bit of luck.  I remember students holding jackets over me to protect me from the rain as I raced to get the last sensor working.  Then we shut the laptop and ran down the hill, trying to beat a huge thunderstorm that started to pelt the area.

collaboration

Desert-FMP Researchers at the Lytle Ranch Preserve

Other challenges of scientific collaboration present organizational hardships.  One of the interesting things about the interdisciplinary science in the Desert FMP project is the complexity of the logistics, and maybe that’s a reason why some people don’t do interdisciplinary projects.  We are finding in order to get good data on the effects of small mammals and plants you need to coordinate when you are sampling small mammals and when you’re sampling plants.  Communicating between four different labs is complicated.  Each of the rainout shelters we use cover an area of approximately 1.5 m2 .  That’s not a lot of space when we have two people interested in soil processes and two people interested in plants who all need to know what’s going on underneath the shelter.  Deciding who gets to take a destructive sample and who can only make measurements that don’t change the system is really hard.  The interesting part of the project where we’re making connections between processes has required a lot of coordination, collaboration, and forward-thinking.

In spite of the headaches, my colleague and I continue to think of ways we can help each other in our research.  Maybe we’re gluttons for punishment, but I think the benefits far outweigh the trouble we’ve had.  For instance, in the above-mentioned Desert FMP project we’ve been able to discover that small mammals are influential in rangeland fire recovery (read about it here).  We only discovered that piece of the puzzle because scientists from differing disciplines are working together.  In our Wasatch Plateau project, my scientist colleague said it was extremely helpful for him to be working with an instrumentation expert who could help him with setup and technical issues.  Also, we’ve been able to secure some significant grants in our Cook Farm Project (you can read about it in an upcoming post) and answer some important questions that wouldn’t have occurred to either one of us, if we hadn’t been working together.  In addition, solving problems that have cropped up in our projects has spurred us on to a new idea for analyzing enormous streams of data in near-real time.  (read about it here).

Download the “Researcher’s complete guide to water potential”—>

Download the “Researcher’s complete guide to soil moisture”—>

Get more information on applied environmental research in our

TDR versus Capacitance or FDR

When we talk with scientists at conferences they often want to know the difference between TDR versus capacitance or FDR.  We’ve written a paper about this in our app guide that has been pretty popular, but it can be difficult to find on our website. Here is an introduction and a link if you are interested in learning more.

TDR Sensor Installation (Giulio Curioni, School of Civil Engineering, Univ. of Birmingham)

TDR Sensor Installation (Giulio Curioni, School of Civil Engineering, Univ. of Birmingham)

Capacitance and TDR techniques are often grouped together because they both measure the dielectric permittivity of the surrounding medium. In fact, it is not uncommon for individuals to confuse the two, suggesting that a given probe measures water content based on TDR when it actually uses capacitance.

TDR

10HS capacitance sensor

With that in mind, we will try to clarify the difference between the two techniques. The capacitance technique determines the dielectric permittivity of a medium by measuring the charge time of a capacitor, which uses that medium as a dielectric. We first define a relationship between the time, t, it takes to charge a capacitor from a starting voltage, Vi , to a voltage V, with an applied voltage, Vf.  Read more….

Watch the webinar

In this webinar, Dr. Colin Campbell discusses the details regarding different ways to measure soil moisture and the theory behind the measurements.  In addition, he provides examples of field research and what technology might apply in each situation. The measurement methods covered are gravimetric sampling, dielectric methods including TDR and FDR/capacitance, neutron probe, and dual needle heat pulse.

 

Take our Soil Moisture Master Class

Six short videos teach you everything you need to know about soil water content and soil water potential—and why you should measure them together.  Plus, master the basics of soil hydraulic conductivity.

Watch it now—>

Download the “Researcher’s complete guide to soil moisture”—>

New Applications in Archeology for TDR Probes Measuring Water Content

Recently, I spent a day at the University of Birmingham in the UK where I talked with Dr. Nicole Metje and researchers in the Civil Engineering department.  They are working on a project called, “Mapping the Underworld,” (Curioni G., Chapman D.N., Metje N., Foo K.Y., Cross J.D. (2012) Construction and calibration of a field TDR monitoring station. Near Surface Geophysics, 10, 249-261) where they are using TDR probes to help locate buried objects that require maintenance.

tdr probes

University of Birmingham Clock Tower

Currently, people use rudimentary tools to poke around and figure out where the buried object is.  A more effective high-tech solution is GPR (Ground Penetrating Radar) that is pulled over the top of the soil and creates a 2D image of permittivity below the ground surface.  The problem is GPR only provides relative depth information and must have ancillary data to produce actual values. To address this issue, their group uses TDR probes (time domain reflectometry) which measure dielectric permittivity to ground truth the GPR.  Using this method they hope to be able to predict the depth to anomalies that are observed in the 2D GPR output.

tdr probes

Sensor Installation Pit

After working on this for some time, the engineers at the University of Birmingham continue to deal with challenges related to TDR signal, interpretation, and maintenance.  One challenge is that TDR systems are complex and power hungry. Thus, the researchers were interested in learning more about soil moisture sensing and different technologies that would help them meet their project goals. My first inclination was to solve their problem with water potential sensors.  Many people who work in environmental applications want to know the fate and distribution of water where water potential is the driver.  Interestingly, this is one of the few cases where people actually do need permittivity measurements (the value used to derive volumetric water content, VWC) instead of water potential because they use the actual permittivity signal to ground truth the GPR.  This realization spawned a four-hour discussion on the frontiers of permittivity measurement in soil and the use of advanced analysis techniques to tease out important soil properties such as bulk density, electrical conductivity, and mineralogy.

I hadn’t given much thought to using soil science instrumentation to locating buried infrastructure.  I’m excited to see what the combination of a new technology like GPR and dielectric measurement can do to help us solve everyday problems like where to start digging.

Download the “Researcher’s complete guide to water potential”—>

Download the “Researcher’s complete guide to soil moisture”—>

Get more information on applied environmental research in our

Near Real-Time Data Analysis

We are entering an era of cheap data.  Sensor technology has advanced to the point where it has become easy to collect large amounts of measurement data at high spatiotemporal resolution.

real-time data analysis

Hydroserver map screen: Using an off-the-shelf open source informatics system like Hydroserver kept us from reinventing what’s already out there, but allowed flexibility to program to our own needs.

We are now to the point where we have gigabytes worth of data on soil moisture, plant canopy processes, precipitation, wind speed, and temperature, but the amount of data is so overwhelming that we are having a difficult time dealing with it. The cost of measurement data is dropping so quickly, people are forced to change from a historical mindset where they analyzed individual data points to the mindset of turning gigabytes of data into knowledge.

real-time data analysis

Because Bioinformatics students are used to working with DNA data, they understand how to write computer programs that analyze large amounts of data in near real-time.

One approach suggested by my colleague Rick Gill, a BYU Ecologist, is to collaborate with bioinformatics students.  Because they are used to working with DNA data, these students understand how to write computer programs that analyze large amounts of data in near real-time.  Rick came up with the idea to tap these students’ expertise in order to analyze the considerable information he anticipates collecting in our Desert FMP Project, an experiment which will use TEROS 21 and SRS sensors to determine the role of varying environmental and biological factors involved in rangeland fire recovery.

Rick and I are predicting that near real-time data analysis will give us several advantages. First, we need readily available information so we can tell that sensors and systems are working at the remote site.  Large gaps in data are common for sites that aren’t visited often, and sensor failures are missed when data are collected but never analyzed.  With our new approach, all data are databased instantly, and the results are visualized as we go.  Not only that, we’ll be able to control what’s being analyzed as we see what’s happening.  We can tell the bioinformatics students what we need as we begin to see the results come in.  If we see important trends, we can assign them to analyze new data that may be relevant right away.

These techniques have the potential to help scientists from all disciplines become more efficient at collection and analysis of large data streams. Although we’ve started the process, we have yet to determine its effectiveness.  I will post more information as we see how well it is working and as new developments arise.

Watch Dr. Gill’s data analysis webinar: Finding Insights in Big Data Sets

Download the “Researcher’s complete guide to soil moisture”—>

Get more information on applied environmental research in our

Complex Scientific Questions Yield Better Science in Desert FMP Project

The Desert FMP project originated from a discussion between pretty divergent scientists: Rick Gill, a BYU ecologist, another scientist who works on soil microbes, a plant physiologist, and a mammalogist who researches small mammals.

Desert FMP

Tree fire in Rush Valley

In an interview Rick said, “We started talking one day about the transformations that have occurred in the arid West over the past 100 years.  One of the things we are really interested in is fire.  How do ecosystems recover after fire? What’s the role of water in rangeland recovery? And the unique piece of this is: what’s the role of small mammals in this process?  We may never have thought of that question, or the complexity of researching how all of our questions work together in a system, if scientists from different disciplines hadn’t decided to collaborate.”

Desert FMP

Rush Valley research site. Five replications with four treatments: burned/unburned and small mammal/no small mammal. What’s interesting for us is that you can see that in the burned plots (the light brown) there are strong differences in the amount of the bright green plant—halogeton—that was present and it is systematically associated with the presence of small mammals. Here is the logic: In the spring, the presence of small mammals suppressed the cheatgrass and to some extent halogeton; in the absence of halogeton, cheatgrass ran wild. The cheatgrass transpired away all of the water and the halogeton that had germinated all died before it could flower.

As the experiment unfolds it is becoming clear that small mammals play a larger role in ecosystem recovery from fire than originally thought.  The scientists have used their observations to hypothesize that small mammals eat the seeds and seedlings of two invasive species. This ends up setting the vegetation along a very different trajectory than when small mammals are absent following fire.  Rick says, “We have discovered this complex but interesting interaction between water, fire, and small mammals. The first year after the fire, a really nasty range forb moved in called halogeton, which is toxic to livestock. Halogeton also accumulates salts in the upper soil profile that will cause failure in native plant germination.  Cheatgrass has also moved in which makes the area more prone to fire as it connects the sagebrush plants with flammable material. But what’s interesting is in treatments where mammals were present, the densities of both halogeton and cheatgrass were much lower than where small mammals were absent.

Desert FMP

Plot water potential comparison using matric potential sensors between Mammal (blue) and no mammal (red) over time. With no mammals to control cheatgrass, it depleted soil water availability below no mammal treatment and consequently halogeten was not able to grow.

 “The other really important thing is that cheatgrass and halogeton have different growth patterns.  Cheatgrass germinates in the Fall.  It reaches peak biomass early in the growing season and then dies off leaving a blanket of dead, highly flammable vegetation.  Halogeton germinates early in the growing season and remains relatively small until early Autumn when it bolts.  These are things that will be really easy to pick up using NDVI sensors, which are sensitive to the amount of green vegetation within the field of view of the sensor.  We are also using a system that we’ve designed to manipulate precipitation input.   This will enable us to connect water availability to the success of two invasive plants that have negative impacts on rangelands.  And with these same treatments we’re going to be able to tease out when in the year and to what extent small mammals are influencing the ecosystem by eating the seeds or the plant and at what stage.”

“Until I saw it in the field, the question of mammals being influential in rangeland fire recovery had never occurred to me.  We only discovered that piece of the puzzle because scientists from differing disciplines are working together.”

Below are two virtual tours of the site:

Get more information on applied environmental research in our

Download the “Researcher’s complete guide to water potential”—>

Solving the Problem of Disappearing Science Lab Technicians

One of the hardest issues university researchers face today is the lack of funding for lab technicians. Although it’s frustrating that universities are no longer able to support this type of personnel, can technology close the gap? This is a question we’ve tried to answer in our Desert FMP project in collaboration with BYU.

lab technicians

Source: Simplyhired.com. Job listings for Science Lab Technicians have decreased 38% from March 2013-March 2014

I was talking to my colleague, Rick Gill, several weeks ago, and he had this to say about the disappearance of the previously indispensable lab technician: “We have fewer people in the lab, and the people we have are more expensive. We need to be deliberate in how we use their time. If we can make the entire system more efficient using technology, we’ll use the people we have in a way that is meaningful. In ecology right now, one of the things that we’re beginning to recognize is that the typical process where the lab tech would go out and take ten samples and average them is not what’s interesting. What’s interesting is when it’s been dry for four weeks, and you get a big rain event. This is because the average for four weeks is really low for almost all processes, but the data three days after it rains swamps the previous four weeks. So the average condition means almost nothing in terms of the processes we’re studying for global change. We need technology to take the place of the technician who would be monitoring the weather and trying to guess when the big events will occur.”

To capture these pulses in the Desert FMP project, we’re using a continuous monitoring system that communicates feedback directly to us as the principal investigators. Using advanced analysis techniques, we can painlessly assure that data are being collected properly and important events are never missed. Although we don’t have a technician, the goals of the project are still being met.

What do you think? How have you dealt with the disappearance of the lab tech?

Download the “Researcher’s complete guide to soil moisture”—>

Get more information on applied environmental research in our

Spectral Reflectance and Water Content in the Wasatch Plateau Experiment

We chose to collaborate with Brigham Young University in an experiment on the Wasatch Plateau in 2009 because a scientist friend of ours had been working in that area the previous five years, and he noticed there were big grazing responses.  The plants growing in the long-term grazed areas were all drought tolerant, while ungrazed plots had plants that were often found only in wetter areas.  The only difference was the fence that kept sheep on one side and not on the other.   The big question was: how does water influence plants in this ecosystem that we understand relatively well? The story had always been the influence of grazers, when in fact, maybe the indirect consequence of grazing was mediated by water.

water

The Wasatch Plateau above Ephraim Canyon, UT, USA.

METER donated some sensors in order to set up an experiment where we changed the amount of water in various plots of land. We had rain exclusion plots, and we had treatments where we collected all incoming rainfall and reapplied it either once a week or every three weeks.   This allowed us to say to what extent this system was controlled by water during the growing season.  To do this, we took measurements with our prototype NDVI Spectral Reflectance Sensor to measure canopy greenness. We also used our prototype volumetric water content sensors to measure soil moisture (this was a few years ago and the sensors were prototypes at the time).  Using these sensors, we found that water is critical in a system people have dismissed as being climate-controlled because it’s at the top of a mountain.

water

A very early prototype of a NDVI sensor measuring canopy greenness in experimental plots on the Wasatch Plateau.

It turns out the amount and timing of precipitation makes a big difference.  We were able to directly connect plant survival, not just to the grazing treatment, but to the actual amount of water that was in the soil. Also, using continuous NDVI data, we were able to look closely at the role of grazing on plant canopies.  When we looked at our NDVI data, we were able to see a seasonal signal, not just a single snapshot sample in time.  So by having the richer data from the data loggers, we obtained a more nuanced understanding of the impact of land use on these important ecological processes.

One of the mistakes we made was failure to include redundancy in the system.  We only had two replicates, so when one of them went down we ended up having just one little case study.  However, that mistake gave us new ideas on how to set up a better system using the right sensors for the job, and it generated a new idea on how to get real-time analysis of data.  In our new Desert FMP project, we have a much better-replicated system where more is invested in the number of sensors that we’re putting out. Each treatment combination will have five to ten water potential sensors.  We are also developing a system where we can analyze data in real-time, so this time we will know when a sensor goes out if a student accidentally kicks it.

 For more details on the Wasatch Plateau Experiment, watch for our published paper that we’ll link to when it comes out.

Download the “Researcher’s complete guide to soil moisture”—>

Get more information on applied environmental research in our