Henry Sintim, PhD student at Washington State University, is investigating whether biodegradable mulches are, in fact, what they claim to be.
Application of plastic mulches conserves water, and helps in weed, pest, and disease control.
He and his research team want to understand what leaches into the soil as the mulches degrade and which ones perform as well as polyethylene-made plastic mulches (PEs) at weed, pest, and disease control.
Plastic Mulch
Application of plastic mulches in agriculture is a common practice by specialty crop producers worldwide. It conserves water, and helps in weed, pest, and disease control, subsequently improving crop yield and quality. Because PE is durable and does not degrade in the soil, you cannot leave it in the field, which ultimately leads to the question of disposal. When PE is buried in the field, it becomes contaminated with soil and can’t be recycled but instead requires transport to a landfill, increasing production costs. Another problem arises when landfill facilities are not available. When this is the case, growers stockpile PE on their farm, where the rain can wash the mulch down to streams and water bodies. Henry Sintim and his team are investigating whether or not biodegradable plastic mulches (BDMs) could be a viable alternative.
The team installs a lysimeter beneath the mulches.
Biodegradable Alternatives
Substituting PE with BDM could alleviate the need for disposal. However, Sintim says the potential impact on agricultural soil ecosystems needs to be assessed before adopting biodegradable mulch for field use. For instance, do biodegradable mulches really degrade? Sintim explains, “By BDM, we mean it is plastic mulch, but it has been made from pure or partial biobased materials. Though there are plastic mulches advertised as biodegradable, none have actually been proven to biodegrade, so the team is examining degradation of different commercial BDM types over time. They have also included an experimental BDM, in which the constituents were specified by the team.”
Sintim is monitoring the degradation of BDM by assessing the material properties and measuring the particle size and surface area via photography: digitizing and analyzing them using Image J software.
There are indications that some of the BDMs are performing well.
How Well Do the Mulches Compare?
Sintim also wants to find out how well BDMs maintain microclimate in comparison to PE. Since soil temperature and moisture content are important parameters that govern chemical reaction rates and microbial activity, and are likely to vary among the different BDM treatments, he is monitoring soil moisture dynamics using soil moisture and temperature sensors installed at 10 cm and 20 cm depths. In addition, the team has installed sensors directly underneath the mulches to measure surface temperature and light penetration. Reduction of light penetration is the attribute that helps plastic mulches to control weeds. The team is also assessing soil quality using the USDA Soil Quality Test Kit.
Sintim says so far one of the commercial BDMs and the experimental BDM had the same yield performance as PE. He adds, “We don’t have final results yet, and there are a lot of variables that could come into the picture. But I will say there is an indication that some of the BDMs are performing well.”
Next week: Find out how Sintim will determine what’s leaching into the soil and another alternative for polyethylene plastic mulch.
Climate change scientists face a particular challenge— how to simulate climate change without contributing to it. Paul Heinrich, a Research Informatics Officer associated with the Southwest Experimental Garden Array (SEGA) remembers looking at the numbers for a DOE project that would have used fossil fuel to measure forests’ response to temperature change. “It would have been very, very expensive in fossils fuels to heat a hectare of forest,” he says.
The alternative is, “to use elevation change as a surrogate for climate change so we could do climate change manipulations without the large energy costs.”
An overview of the SEGA sites using elevation change as a surrogate for climate change. For more information on these sites, visit http://www.sega.nau.edu/. Photo credit Paul Heinrich
By monitoring organisms across a temperature gradient it is possible to identify genetic variation and traits within a species that could contribute to a species survival under projected future climates.
Control and Monitoring Infrastructure
SEGA is an infrastructure project started in 2012 after researchers at Northern Arizona University’s Merriam-Powell Center for Environmental Research were awarded a $2.8 million dollar NSF grant with a $1 million match from NAU. Consisting of ten fenced garden sites for genetics-based climate change research, SEGA is set on an elevation gradient from 4000 to 9000 feet in the Southwestern United States. Each SEGA site has an elaborate data collection and control system with meteorological stations and site-specific weather information. Custom-engineered Wireless Sensing Actuating and Relay Nodes (WiSARDs) send data packets to a hub which then send the data back to a centralized server.
Because there is inherent moisture content variability from site to site, volumetric water content and soil water potential sensors have been installed to monitor and maintain moisture levels. If there is a change in soil moisture at one site, soil sensors will detect the difference. Software on the server notes the difference and sends a signal to the other sites, turning on irrigation until the soil moisture matches across sites.
An illustration of SEGA’s cyberinfrastructure and data management system. Photo credit Paul Heinrich.
Having such an elaborate infrastructure creates an opportunity for researchers looking to conduct climate change research. By offering access to the pre-permitted SEGA sites, the hope is that research will generate much-needed data for climate projections and land management decisions.
When asked if the data stream was overwhelming to manage Heinrich said, “Well, not yet. We are just getting started. The system is designed for what SEGA is expected to look like in ten years, where we expect to have 50 billion data points.”
Research Considerations
Climate change projections show temperatures increasing rapidly over the next 50 to 100 years, bringing drought with it. The impact of these changes will be dramatic. Temperature and drought tolerant species will survive, those that are not will die, drastically changing the landscape in areas that are currently water stressed. Pests like the pine beetle and invasive species like cheatgrass will do well in a drier environment where water-stressed natural species will not be able to compete.
Soap Creek, AZ from above. With climate change projections it is likely that more land will become marginal. Photo credit Paul Heinrich.
“Foundational species,” or species that have a disproportionate impact on the ecosystem, are the primary focus of the research efforts at SEGA sites. These are the species that drive productivity, herbivore habitat, and carbon fixation in the ecosystem. Unlike forests in other parts of the United States, forests in the Southwest can be dominated by one or two species, which makes potential research subjects easier to identify.
Genetic Variance
Amy Whipple, an Assistant Professor in Biology and the Director of the Merriam-Powell Research Station who oversees the day-to-day activities at SEGA, has been conducting some of her own research at the garden sites. Whipple has studied Piñon Pine, Southwestern White Pine, and has a proposal to study Cottonwood in process.
Whipple says that models currently suggest that Piñon Pine will be gone from Arizona within the next 50 years, adding that the models do not take into account possibilities for evolution or genetic variance that might help the Piñon survive. Her research is largely asking, will trees from hotter, drier locations have a better chance of surviving climate change? “We’re trying to do that with a number of different species to look for ways to mitigate the effects of climate change in the Southwest.”
Researchers documenting a Piñon Pine. Photo credit Paul Heinrich.
In some of her research on Piñon Pine, it was discovered that four different species were grouped morphologically and geographically from southern Arizona to Central Mexico. While this suggests that the divergence of species has occurred, it also suggests a low migration rate for these tree species. Migration rates of drought and temperature tolerant species is an important consideration when modeling for a future climate. If the migration of genetically adapted species cannot keep up with climate, the land could become marginal as a foundational species dies off.
Climate Change Predictions and Considerations
In the Southwest, there are entire forests that could become grassland in 50 years because the genetic characteristics of the foundational species currently in those regions will not adapt to higher temperatures and drought stress. But what does this mean from a land management perspective?
Ponderosa pine trees, a foundational species in some area of the Southwestern United States.
Environmental conservationists maintain that we should protect the unique species that are in a place and that introducing other organisms or genetic material would be an ethical violation. Environmental interventionists make the argument that climate change has been caused by humans, so we have lost the option of remaining bystanders.
Research, Land Management and Policy
Paul Heinrich says that the route we take to manage the land will depend on our end goals. “Places that have trees now, if you want them to have trees 50 years from now, you are going to have to do something about it. The trees that are on the landscape right now are locally adapted to the past climate. They are not necessarily adapted to the future climate. They are probably maladapted to the future climate.”
To be clear, SEGA’s goal is not to promote or implement assisted migration. Instead, Amy Whipple says, SEGA can test what the effects of assisted migration might be. “In a smaller experimental context, we’re asking: how will these plants do if we move them around? What will happen to them if we don’t move them around?’” The goal is to provide decision makers with the data they need to make informed decisions about how to manage the land.
The Arboretum Meadow in Flagstaff, AZ. Home of one of the SEGA research sites. Photo credit Paul Heinrich.
Whipple’s own view is that we may no longer have the option of doing nothing. “Unless major changes are made for the carbon balance of the planet, keeping things the same is not a viable option. Managing for a static past condition is not viable anymore.”
Remaining Questions
Both Heinrich and Whipple acknowledge that these are inherently difficult questions. Ultimately the public and land managers must make these decisions. In the meantime, data from SEGA research may help ensure better predictions, better decisions, and better outcomes.
Innovative soil scientist, John Buck, and his team have discovered that green roofs have more capacity than people imagined (see part I). Below are some of the challenges he sees for the future, and the type of measurements he suggests researchers take, as they continue to validate the effectiveness of these urban ecosystems.
A green roof is essentially a garden on a roof, but rather than growing plants in soil, installers use a synthetic substrate made of expanded shale, expanded clay, crushed brick, or other highly porous, lightweight material.
New Challenges for Green Roofs
Green roof results are promising, but they present a new challenge: making sure the plants have enough water. The crux of the challenge is that the lightweight, expanded shale/clay substrate material, the standard in green roof design, does a good job of soaking up the water, but has some peculiar properties that are unlike typical soils. Specifically, the expanded shale and expanded clay media tend to be dominated by sand and fine gravel-sized particles that provide a high proportion of macropores, but the interior porosity of the large particles is dominated with micropores. That pore size distribution leads researchers to two important questions— How much water will be readily available for plant growth? And, will the unsaturated hydraulic conductivity be adequate to avoid starving the roots under high-evaporative demand by allowing water to flow to roots from the bulk soil? These are critical questions as green roof technologies continue to evolve.
Researchers wonder, will the unsaturated hydraulic conductivity be adequate to avoid starving the roots under high-evaporative demand.
Measurements Required for Green Roof Validation
Still, Buck has learned a great deal from his work. Considering the wild spatial distribution of summer storms, quantitative green roof performance studies require that rainfall be measured locally. Monitoring of soil volumetric moisture content measurements in concert with rainfall and soil lysimeter measurements of drainage, reveal the degree of total and capillary saturation, drainage rate, and porosity available for storage. Soil water potential sensors, placed within the capillary fringe of water ponded over subsurface drainage layers, can provide useful insights regarding the dryness of the drainage layer and overlying soil, as well as the available storage of stormwater within the drainage layer.
Direct measurement of soil drainage using lysimeters is a key supplemental measurement on green roof performance quantification projects because there is an unmeasured component of water storage where drought-resistant alpine succulents (typically Sedum species) are used on green roofs. The Sedum plants can absorb up to 10 mm of rainfall equivalent in their plant tissues.
Measurement of soil drainage using lysimeters is a key supplemental measurement on green roof performance quantification projects.
Other Projects and Future Plans
At ground level, Buck is quantifying the performance of intensive stormwater infiltration areas known as rain gardens, bioretention areas, or more generically, infiltration-based stormwater best management practices (Infiltration-based BMPs). When monitoring infiltration-based stormwater BMPs, Buck has used similar tools to those used on green roofs, but has added water-level sensors and piezometers. Buck has found that ancillary measurements of electrical conductivity, often available on water content sensors, along with surface and pore water sampling, can be used to document transformations taking place in infiltration systems. These measurements now combine to show that green roofs and infiltration-based BMPs are indeed making a difference to urban environments and contributions to CSOs. The challenge now is how to implement this technology more widely. But, with the validation now in hand, that job should be quite a bit easier.
Green roofs are being built in large cities to provide stormwater management, reduce the urban heat island effect, and improve air quality—but are they effective? John Buck, an innovative soil scientist based in Pittsburgh, Pennsylvania, has been trying to quantitatively answer this question in many different cities using soil monitoring equipment in order to determine the efficacy and best types of green infrastructure for managing stormwater.
A green roof installation site at the Allegheny County Office Building in Pennsylvania.
Why Green Roofs?
In older cities, stormwater runoff is typically combined with sewage flows, and these combined waters are treated at a sewage treatment plant during dry weather and light rain events. Unfortunately, during more substantial storms (sometimes just a few mm of rain) the combined flows exceed the ability of the sewage treatment plant, and are discharged without treatment to surface waters as “combined sewage overflows” (CSOs). One of the ways to mitigate CSOs is to capture and store stormwater to keep it out of the combined sewer.
A green roof is essentially a garden on a roof, but rather than growing plants in soil, installers use a synthetic substrate made of expanded shale, expanded clay, crushed brick, or other highly porous, lightweight material with high infiltration rates. During a storm event, water will soak into the air-filled pore space in the substrate, which acts like a sponge to soak up the rain. Excess water will flow into a subsurface drainage layer and will leave the roof garden via existing roof drains. Because a substantial fraction of the stormwater is stored in the substrate, it can later dissipate through evapotranspiration instead of contributing to stormwater volume and CSOs.
Researchers are using soil moisture sensors for measuring temperature, bulk electrical conductivity and volumetric water content in green roofs and green infrastructure.
Finding Answers
Designers and regulators want to know how well green roofs work and if they are being over-engineered. They want answers to questions such as: “What sort of substrate should I be using? What type of plants can survive green roof conditions? Will I need to irrigate the green roof when there are no storms to water the plants?” and, “Will the green roof work as well during a one-inch storm that occurs over a half hour versus a five-inch storm that occurs over five days?”
Buck is using soil lysimeters and modified tipping bucket rain gauges to measure the quantity, intensity, and quality of water coming into and going out of the green roofs. He also tracks weather parameters and calculates daily evapotranspiration of landscapes. Using soil sensors, he measures electrical conductivity (dissolved salts), volumetric water content, and temperature. He has installeddata loggers that send data to the web via GSM cellular connection, allowing stakeholders access to the data in real-time. This data telemetry provides additional data security, immediately updated results, instant feedback of system problems, and an easy way to share data with others.
Visualized data of the 87% annualized runoff reduction at Phipps Conservatory green roof site in Pittsburgh, PA.
What Has Been Learned?
Buck discovered that green roofs have much more capacity than people ever imagined. At The Penfield Apartments in St. Paul, Minnesota, the green roof retained enough water to reduce runoff to about half of a conventional roof, and the peak intensity of the runoff was about one-quarter of what it would have been without the green roof. At Phipps Conservatory in Pittsburgh, there was an 87% annualized runoff reduction and almost no runoff from typical summer rain events. Buck comments, “Interestingly, on the Penfield project, we expected better hydrologic performance where soils were thicker, but there was no difference, or results were slightly the reverse of expectations. That reversal was likely due to the confounding influence of irrigation, which was probably non-uniform and not metered or measured by the rain gauge.”
Next week:Read about some of the challenges John Buck sees for the future, and what kind of measurements he suggests researchers make, as they continue to validate the effectiveness of these urban ecosystems.
Due to controversy over the growing number of high capacity wells in the Wisconsin Central Sands, University of Wisconsin PhD student, Mallika Nocco, is researching how agricultural land use, irrigation, and climate change impact the region’s water-energy balance (see part I). This week, read about her challenges installing lysimeters below the root zone, how she used a GPS system that can find the lysimeters within a half-inch of accuracy, and her surprising conclusions.
This relatively small ecological region has gone from 60 high capacity wells in 1960 to over 2,500 today.
Below the Root Zone
Nocco says getting the lysimeters below the root zone was a major challenge. “We tried a couple of things, but we settled on installing all the lysimeters with an 18-inch auger that would drill a hole slightly bigger than the whole lysimeter. We dug an 80 cm trench to the top of the monolith zone. Then, we pounded the drain gauge divergence control tube to 1.4 m to obtain an intact monolith, wherever it was possible to do so. We also stratified soil moisture sensors at 10, 20, 40, and 80 cm. We used heavy equipment to slowly lift out the monolith, dig out the soil below, and place it back in, keeping track of all of the different soil horizons, and backfilled as close to the bulk density as we could.”
Passive capillary lysimeter installation
Finding the Lysimeters with GPS
Typically, scientists bury lysimeters close to the edge of the field so they are easy to locate, but Nocco was concerned that they would prejudice their data due to the donut effect of center pivot irrigation: more irrigation hits the center of the field with less irrigation toward the edges. She comments, ”When I installed the first ten lysimeters, I had not yet come up with a way to find everything. Those instruments are all about 15 meters from the field edge so that I could triangulate measurements and find them during cultivation. But then I met an extension scientist at the university who had access to an RTK GPS system, which can locate instrumentation within a half-inch of accuracy. With his help and training, we were able to install the rest of the lysimeters at more random spots throughout the field.”
Nocco was concerned that they would prejudice their data due to the donut effect of center pivot irrigation.
Surprising Conclusions
Nocco says that ET and differences in crop physiology do not explain or account for all of the variability that she saw in groundwater recharge. Her team did a particle size analysis on the soils adjacent to the lysimeters, and she comments, “We thought that the greater the relative sand content in the soils, the more recharge we would have seen, but what we are seeing is the opposite. The particle size analysis reveals a negative linear correlation between potential recharge and sand content. The more silt there is in these lysimeters, the more volume of recharge. What I’m curious about now is if we’re seeing a greater volume of recharge in the siltier spots from flux convergence. I’m trying to obtain the time series data from the pressure transducers to see if maybe the sandier areas had less potential recharge, but perhaps drained faster. I have seen a correlation between antecedent soil moisture content and particle size (with no correlation based on crop type). So it also looks like the siltier soils are holding more water when the rain comes through.”
What’s Next?
Eventually, Nocco plans to use field-generated estimates of groundwater recharge and ET to parameterize and validate a dynamic, agroecosystem model, Agro-IBIS, simulating hydrological responses to climate and land use changes over the past 60 years. Nocco will then share the water-energy budgets and water quantity/climate simulations with stakeholders in the Wisconsin Central Sands area.
Due to controversy over the growing number of high capacity wells in the Wisconsin Central Sands, University of Wisconsin PhD student, Mallika Nocco, is researching how agricultural land use, irrigation, and climate change impact the region’s water-energy balance. She and her team have uncovered some surprising results.
A class 1 trout stream has sufficient natural reproduction to sustain populations of wild trout at or near carry capacity.
Water Use Debate
There are class 1 trout streams in the Central Sands region, and some people worry that the increasing number of high capacity wells used for agriculture will reduce the water levels in those streams. “Lake Huron has lost about 11 feet of water since 2000,” says one resident of the Central Sands area, “and water levels are continuing to drop.” In 2008, the small well he used to pump drinking water went dry, and he blames the high capacity wells.” (Aljazeera America) On the other side of the debate, agriculture irrigated by these wells is extremely valuable to the state, and growers have taken quite a bit of time to understand the water cycle and their role in it. You can read about their water management goals and accomplishments here.
Updating Former Research
Irrigated agriculture wasn’t prevalent or profitable in the Wisconsin Central Sands until groundwater irrigation with high capacity wells became feasible in the 1950s. Since then, this relatively small ecological region has gone from 60 high capacity wells in 1960 to over 2,500 today.
Mallika Nocco is studying potential groundwater recharge from irrigated cropping systems that use the wells, hoping to understand if the irrigation water is lost or returned to the groundwater. She says, “Until now, we’ve been relying on models validated by two lysimeters in the 1970s. Champ Tanner (one of the fathers of environmental biophysics) designed the weighing lysimeters, and they were very accurate, but we wanted to do a larger scale study with multiple crops to get a handle on interannual variability and to improve our understanding of recharge in the region so we can do a better job of managing irrigation and groundwater.”
Lysimeter installation into actively managed fields presented challenges that the research team had to overcome.
Measuring Recharge
Nocco used twenty-five drain gauge lysimeters to capture vadose zone flux under potato and maize cropping systems. She monitored soil water (and temperature) flux by stratifying water content sensors from the soil surface to a depth of 1.4 meters. She also estimated evapotranspiration (ET) using a porometer to measure stomatal conductance, in addition to obtaining micrometeorology, leaf area index, and gas exchange measurements.
Nocco and her team had to put their sensors in to avoid cultivation, so they extended the drain gauge PVC that comes up to the soil surface and removed it any time there was major fieldwork, whether it was tillage or planting, so that the area over the lysimeter got the same treatment as the rest of the agricultural fields.
Below the Root Zone
Nocco says getting the lysimeters below the root zone was a challenge. Next week, read about how she solved that challenge, how she used a GPS system to find the lysimeters within a half-inch of accuracy, and about her surprising conclusions.
Dr. Lauren Hallett, researcher at the University of California, Berkeley, recently conducted a study testing the importance of compensatory dynamics on forage stability in an experimental field setting where she manipulated rainfall availability and species interactions. She wanted to understand how climate variability affected patterns of species tradeoff in grasslands over time and how those tradeoffs affected the stability of things like forage production across changing rainfall conditions.
Species tradeoffs could help mitigate the negative effects of climate variability on overall forage production.
Species Tradeoff
A key mechanism that can lead to stability in forage production is compensatory dynamics, in which the responses of different species to climate fluctuations result in tradeoffs between functional groups over time. These tradeoffs could help mitigate the negative effects of climate variability on overall forage production. Dr. Hallett comments, “In California grasslands, there’s a pattern that is part of rangeland dogma, that in dry years you have more forbs, and in wet years you have more grasses. I wondered if you could manage the system so that both forbs and grasses are present in the seed bank, able to respond to climate. This would perhaps buffer community properties, like soil cover for erosion control and forage production in terms of biomass, from the effects of climate variability.”
In areas experiencing moderate grazing, there was a strong species tradeoff between grasses and forbs.
Manipulating Species Composition
Dr. Hallett capitalized on the pre-existing grazing manipulation that her lab had done over the previous four years. The grazing she replicated for this study was experimentally controlled, making it easier to ensure consistency. She built rainout shelters where she collected the water and applied it to dry versus wet plots. She also manipulated species composition, allowing only grasses, only forbs, or a mix of the two. These treatments allowed her to study changes in cover and biomass.
Hallett used soil moisture probes and data loggers to characterize the treatment effects of this experiment and to parameterize models that predict rangeland response to climate change. She says, “I wanted to verify that my rainfall treatments were getting a really strong soil moisture dynamic, and I found the shelters and the irrigation worked really well.” Along with above-ground vegetation, she collected soil cores and looked at nutrient differences in conjunction with soil moisture. Since her field site is located within the Sierra Foothills Research and Extension Center, Dr. Hallett was able to rely on precipitation data that was already measured on-site.
Results
Dr. Hallett found that in areas experiencing moderate grazing, there was a strong species tradeoff between grasses and forbs. She comments, “I had a seedbank that had both functional groups represented, and those tradeoffs did a lot to stabilize cover over time.”
When Dr. Hallett replicated the experiment in an area that had a history of low grazing, she found that the proportion of forbs wasn’t as high in the seedbank. As a consequence, there was a major loss of cover in the dry plots. She explains, “When the grass died, there weren’t many forbs to replace it, and you ended up with a lot of bare ground. The areas that were lightly grazed had more litter, so initially, the soil moisture was okay, but as the season progressed into a dry condition and the litter decomposed, there wasn’t enough new vegetation to stabilize the soil.” As a result, Dr. Hallett thinks in low-grazed areas it’s important to have an intermediate level of litter. She says, “You need enough litter to increase soil moisture, but not so much that it would suppress germination of the forbs because as the season progresses and gets really dry, if you don’t have forbs in the system, you lose a lot of ground cover.”
Surprises Lead to A New Study
Dr. Hallett was surprised that within her three treatments there seemed to be differences in when the functional groups were drying down the soil. This inspired new questions, leading her to use her dissertation data to generate a larger grant through the USDA. Her new study will perform extensive rainfall manipulations to measure the effects of early-season versus late-season dryout, and vary species within those parameters. She says, “One of the reasons you have grass years versus forb years is the timing of rainfall. For instance, if you have a really dry fall, you tend to have more forbs because their seedlings are more drought resistant. Conversely, if you have a wet fall, you tend to see more grasses because you have continual germination throughout the season. So, the timing of rainfall matters in terms of what species are in the system. We are going to look at the coupling between the species that gets selected for the fall versus what would be able to grow well in the spring, and we will be studying how that affects a whole range of things such as ground cover, above-ground production for forage, below-ground investment of different functional groups, and how these things might relate to nutrient cycling and carbon storage.”
You can read more about Dr. Hallett’s rangeland research and her current projects here.
Dr. Stuart Campbell, professor of Biomedical Engineering at Yale University has been toying with the idea of using soil moisture sensors to measure tissue edema in human subjects.
Tissue edema occurs when too much fluid leaks from your capillaries into your tissue.
He says he got the idea from Dr. Ken Campbell, former professor of Bioengineering at Washington State University: “I was explaining to Dr. Campbell about the sensorsMETER makes, and he pointed out that there are many diseases where you might want to measure someone’s tissue edema, and it would be interesting to see if you could use a soil sensor in a wearable device to help doctors monitor swelling in their patients, much like a heart monitor monitors heart activity.”
Tissue Edema:
Tissue edema occurs when too much fluid leaks from your capillaries into your tissue. Capillaries, the smallest blood vessels in your body, are somewhat leaky, allowing the exchange of nutrients and waste between the tissue and the blood. The fluid that surrounds the blood cells is free to exchange across the capillaries, and edema will occur when too much fluid leaks out of the circulatory system into the tissue. Edema can be caused by things like heart disease, pregnancy, or standing on your feet all day.
What Makes the Fluid Leak?
In soil, water moves from high water potential to low water potential. Similarly, there are forces inside the circulatory system that cause the transfer of fluid between capillaries. Your blood vessels have a certain amount of pressure that is generated by your heart. If your blood pressure goes up, it can cause edema. Dr. Stuart Campbell says, “The actual fluid pressure is part of what decides how much fluid is pushed out, but it’s not that simple. Your blood has large proteins that are too big to get out of the capillary. That means the more water that leaves the capillary and moves into the tissue, the more concentrated those proteins become, which lowers the water potential (or osmotic potential) of the blood. This delicate balance is what prevents too much water from leaking out. However, if you have a disease that tips this balance, either through high blood pressure or a condition that allows those proteins to leak out of the capillary, edema would occur because you don’t have the osmotic potential pulling the water back into the capillary and keeping the proper balance.”
Dr. Campbell thought it would be interesting to figure out if he could monitor the edema of heart tissue during one of the procedures.
The Heart Experiment:
Dr. Campbell decided to see if a soil sensor would work to measure animal tissue when he was working as a summer student in the Visible Heart Lab at the University of Minnesota. Campbell says, “Similar to a human heart transplant, this lab is able to keep pig hearts alive outside the body. The problem, however, is that they use a manmade solution instead of blood, and that imitation blood is not ideal. If the composition of the fluid is not perfectly adjusted, you can have problems with your experiments. I thought it would be interesting to figure out if we could monitor the edema of the heart tissue during one of the procedures. I hooked up the soil probe and used it in one experiment where I put it in contact with the heart while it was beating. There was, in fact, a change in output of that signal during the experiment. But, because I only got one chance at it, it was inconclusive as to whether this was indicative of an imbalance in the composition of our artificial blood substitute.”
An Anecdotal Experiment:
Still curious to see if the idea would work, Dr. Campbell decided to try one more experiment: this time on his wife who was experiencing edema symptoms after childbirth. He says, “It occurred to me that this was an opportunity to try out the soil moisture probe one more time to measure tissue edema. So each day, I would measure her ankles, putting the probe in flat contact with her skin while tightening a strap gently.” Dr. Campbell says he watched the swelling go down as the numbers on the probe got smaller, and comments, “It was anecdotal evidence that at least in extreme cases, you might be able to get the soil probe to work. But I still have questions, such as, how would you make sure that the probe was always touching the skin in the same way? And, if the person got sweaty, would that change the soil probe reading?”
There are millions of people in this country who have heart failure.
Why the Experiments Should Continue:
Though Dr. Campbell hasn’t had time to pursue the experiment further, he feels that if the idea works, it has the potential to improve lives and save our nation billions of dollars. He says, “There are millions of people in this country who have heart failure. Maybe they’ve had a blockage in one of their coronary arteries, or perhaps their heart is worn out because of age. You can tell when someone is in heart failure because when they lie down to go to sleep at night, all that fluid makes its way slowly from the ankles, through the legs, the torso, and eventually into the chest. The problem is that the lungs are very delicate, and when you have edema in the lungs, it’s almost like you have pneumonia. This type of sensor could be an easy way for people to monitor themselves and manage their fluid intake and diet after they get home from the hospital.” Dr. Campbell says this helps the economy because if people don’t manage their fluids, they have to return to the hospital so they can be supervised to eat correctly and regain the proper fluid balance. This ends up costing the economy billions of dollars unnecessarily. He concludes, “Perhaps people just need to follow instructions, but it’s possible with better monitoring that the situation can be improved.”
Understanding the amount of drainage that comes out of the bottom of the root zone and infiltrates into groundwater recharge is a very difficult measurement to do well. Drain gauges do a good job of it but on a small scale. Large lysimeters do an even better job, but are extremely expensive and complex. There is an economical alternative, however, called the salt balance approach to measuring drainage.
Soil profile underneath canola
The Salt Balance Approach
Since the majority of non-fertilizer salts in the soil solution don’t get taken up by plants, this salt can be used in soil as a conservative tracer. This means that whatever salt is applied to the soil through rainfall or irrigation water is either stored in the soil or leaches through the profile with the soil water, enabling us to use conservation of mass in our salt balance analysis. The electrical conductivity of water (ECw) is directly proportional to the salt concentration, so ECw can be used in place of salt concentration in this analysis. If you measure the EC of the water that’s applied to the soil, either through irrigation or precipitation, as well as the EC of the water that’s coming out of the bottom of your profile, then you can calculate what fraction of the applied water is being transpired by the plants, and what fraction is draining out of the bottom. This method is useful for measuring water balance at field sites.
To illustrate this concept, let’s work through a simple example. A particular field received 40 cm of water through precipitation and irrigation. The average ECw of the precipitation and irrigation water is 0.5 dS/m. Measurements of ECw draining from the soil profile below the root zone indicate an ECw of 2.0 dS/m. The drainage or leaching fraction can be easily calculated as :
The amount of water drained can also be easily calculated as:
Leaching fraction * applied water = 0.25 * 40 cm = 10 cm
Measuring Pore Water EC (ECw)
One challenge to this approach is the measurement of water electrical conductivity itself. Bulk EC is a relatively simple measurement, and several types of soil water content sensors measure it as a basic sensor output. However, the electrical conductivity of water, called pore water EC (ECw), is more complex. Pore water EC requires that it be either estimated from the bulk EC and soil water content or that a sample of pore water be pulled from the soil matrix and measured. When estimated, pore water EC can contain considerable error. In addition, removing a water sample and measuring the pore water EC is not easy.
To learn more about measuring EC, read our EC app guide.
Forests along the California coast and offshore islands experience coastal fog in summer, when conditions are otherwise warm and dry. Since fog-water inputs directly augment water availability to forests during the dry season, a potential reduction of fog due to climate change would place trees at a higher risk of water stress and drought-induced mortality. Dr. Sara Baguskas completed her Ph.D. research in the geography department at UC Santa Barbara on how variation in fog-water inputs impact the water relations of a rare, endemic tree species, Bishop pine, located on Santa Cruz Island in Channel Islands National Park. The goal of her study was to enhance our ability to predict how coastal forests may respond to climate change by better understanding how fog-water inputs influence the water budget of coastal forests.
Dr. Baguskas’ study seeks a better understanding of how fog-water inputs influence the water budget of coastal forests.
Fog Manipulation
Santa Cruz Island supports the southern extent of the species range in California, thus it is where we would expect to see a reduction in the species range in a warmer, drier, and possibly less foggy future. To advance our mechanistic understanding of how coastal fog influences the physiological function of Bishop pines, Dr. Baguskas conducted a controlled greenhouse experiment where she manipulated fog-water inputs to potted Bishop pine saplings during a three-week drydown period. She installed soil moisture (VWC) sensors horizontally into the side of several pots of sapling trees at two different depths (2 cm and 10 cm) and exposed the pines to simulated fog events with a fog machine.
In one group of plants, Baguskas let fog drip down to the soil, and in another treatment, she prevented fog drip to the soil so that only the canopies were immersed in fog. She adds, “Leaf wetness sensors were an important complement to soil moisture probes in the second treatment because I needed to demonstrate that during fog events, the leaves were wet and soil moisture did not change.” Additionally, Baguskas used a photosynthesis and fluorescence system to measure photosynthetic rates in each group.
The fog events had a significant, positive effect on the photosynthetic rate and capacity of the pines.
Results
Dr. Baguskas found that the fog events had a significant, positive effect on the photosynthetic rate and capacity of the pines. The combination of fog immersion and fog drip had the greatest effect on photosynthetic rates during the drydown period, so, in essence, she determined that fog drip to the soil slows the impact of drydown.
“But,” she says, “when I looked at fog immersion alone, when the plant canopies were wet by fog with no drip to the soil, I also saw a significant improvement in the photosynthetic rates of these plants compared to the trees that received no fog at all, suggesting that there could have been indirect foliar uptake of water through these leaves which enhanced performance.” An alternative interpretation of that, Baguskas adds, is that nighttime fog events reduced soil evaporation rates, resulting in less evaporative loss of soil moisture.
Dr. Baguskas says her “canopy immersion alone” data are consistent with other research: Todd Dawson, Gregory Goldsmith, Kevin Simmonin, Carter Berry, and Emily Limm have all found that when you wet plant leaves, it has a physiological effect, suggesting the plants are taking water up through their leaves and not relying as much on soil moisture. (These authors performed different types of experiments, but their papers serve as reference studies). Baguskas says, “My results suggest that is what’s going on, but it’s not as definitive as other studies that have actually worked on tracking the water through leaves using a stable isotope approach.”
Lessons Learned
Though Dr. Baguskas did not monitor soil temperature in this study, she says that in the future, she will always combine temperature data with soil moisture data. She comments, “Consistently, the soil moisture in the “canopy-immersed only” plants was slightly elevated over the soil moisture in the control plants. It made me wonder if this was a biologically meaningful result. Does it support the fact that if plants are taking up water through their leaves, they don’t rely on as much soil moisture? Or did my treatment change soil temperature, and is that having a confounding effect on my results? What I’ve learned from this, is that in the future I will always use soil probes with temperature sensors because you may not know until you see your results if temperature might be important.”
Future Fog Studies
Baguskas is a USDA-NIFA postdoctoral Research Fellow working with Dr. Michael Loik in the Environmental Studies Department at UC Santa Cruz. She continues to study coastal fog, but now in strawberry fields. Her current research questions are focused on integrating coastal fog into water-use decisions in coastal California agriculture. She loves the work and continues to rely on soil moisture sensors to make meaningful and reliable environmental measurements in the field and greenhouse.
